iBiomagazine

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Haptoglobin (Hpt) in serum, plasma and other biological fluids.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Haptoglobin (Hpt) in serum, plasma and other biological fluids.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Haptoglobin (Hpt) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HP in the samples is then determined by comparing the OD of the samples to the standard curve.

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HP in the samples is then determined by comparing the OD of the samples to the standard curve.